HYDROACOUSTICS
ANNUAL JOURNAL
START NEW VOL 20 SEARCH STATISTICS PAS - GDANSK DIVISION

Rat cancer cells necrosis induced by ultrasound

pp. 179-186, vol. 17, 2014

Wojciech Secomski
Institute of Fundamental Technological Research, Polish Academy of Sciences, Warsaw, Poland

Krzysztof Bilmin
Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland

Tamara Kujawska
Institute of Fundamental Technological Research, Polish Academy of Sciences, Warsaw, Poland

Andrzej Nowicki
Institute of Fundamental Technological Research, Polish Academy of Sciences, Warsaw, Poland

PaweĊ‚ Grieb
Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland

Key words: sonodynamic therapy; cytotoxic activity

Abstract: Sonodynamic therapy is the ultrasound dependent enhancement of the cytotoxic activities of certain drugs called sonosensitizers. The study of therapeutic efficacy of ultrasound is always preceded by in-vitro tests. In this work, two in-vitro sonication procedures were compared. One with the transducer positioned bellow the cell colony, radiating upward, with standing wave reflected from the water-air surface, the second, in the free field conditions. Efficiency of the cancer cells necrosis caused by ultrasound was compared with acoustical field intensity I SPTA measured by a hydrophone. The standing wave conditions effectively increased the intensity of the ultrasonic wave at the level of cells. To achieve 50% of cell viability, the intensity I SATA , decreased from 5.8 W/cm2 to 0.3 W/cm2 . In summary, sonication in the standing wave conditions can effectively and reproducibly destroy cells by ensuring the sterility and without the risk of overheating.

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© Polish Acoustical Society - Gdansk Department, Polish Academy of Sciences. This work is licensed under the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported. (CC BY-NC-SA 3.0)