Abstract: This paper describes results of enhanced version, of the macrosounding method to integrate acoustic data with environmental and biological parameters in a time and space scale, for use in marine ecosystem analyses. Fish distributions are shown by a vari-coloured topographical space, or time based, matrix of volume back-scattering strength (s_v). The matrix is calculated for one or the series of surveys for each distance unit (ESDU) in standardized slices of insonified volume. Each s_v visualization is accompanied by a parallel cross-section of the same space unit, which provides a distribution of the selected environmental parameter measured during the same survey. Charts of such parameters are produced with the same procedures as for s_v. This presentation of multi-disciplinary data greatly improves recognition of time and space gradients. Analyses of data from multi-dimensional matrix calculated for individual transect improve interpretation and modeling of ecosystem characteristics. Application of the method was illustrated by examples, showing its practical use for data from 1994-2001 in the southern Baltic, collected during the autumn ICES acoustic surveys.